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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
08/12/2023 |
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Data da última atualização: |
13/12/2023 |
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Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
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Autoria: |
LACORTE, C. C.; FERREIRA, A. L.; MURAD, A. M.; CUNHA, N. B. da; PINHEIRO, P. V. |
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Afiliação: |
CRISTIANO CASTRO LACORTE, Cenargen; AMANDA LOPES FERREIRA, bolsista CNPAF; ALINE MELRO MURAD, UFSC; NICOLAU BRITO DA CUNHA, UNB; PATRICIA VALLE PINHEIRO, CNPAF. |
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Título: |
Seed-based production of recombinant proteins. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
In: KOLE, C.; CHAURASIA, A.; HEFFERON, K. L.; PANIGRAHI, J. (ed.). Tools & techniques of plant molecular farming. [S.l.]: Springer Singapore, 2023. Cap. 6. |
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Páginas: |
Cap. 6, p. 185-208. |
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DOI: |
https://doi.org/10.1007/978-981-99-4859-8_6 |
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Idioma: |
Inglês |
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Conteúdo: |
The use of plants to produce recombinant proteins has become a promising alternative to current expression systems based on microorganisms and cell cultures. Producing recombinant proteins in plants presents advantages such as reduced upstream costs, lower probability of infection by mammals’ pathogens, and easy scale-up production. Furthermore, plants can make posttranslational modifications and express large and complex proteins. Seeds stand out for their intrinsic characteristics among the plant tissues that can be used for recombinant protein production. In seeds, recombinant proteins are stored in protein storage vacuoles in the endosperm cells. They can be stably maintained for 4–6 years at room temperature without significant loss of protein and biological activity. Progress in methods for genome editing, alteration of the glycosylation pattern of the recombinant proteins produced, and use of protein fusions to aid downstream processes are some trends that shall contribute further to make molecular farming an attractive option for recombinant protein production. This chapter discusses the potential of seeds as a platform for producing recombinant proteins, across the scale-up of the production systems, basic approaches for the purification of recombinant proteins from plant cells, and biosafety issues |
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Palavras-Chave: |
Foreign gene expression; Fusion proteins; Heterologous protein production; Plant molecular farming; Recombinant protein purification. |
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Thesaurus Nal: |
Biosafety; Glycosylation. |
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Categoria do assunto: |
--
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02273naa a2200277 a 4500
001 2159472
005 2023-12-13
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/978-981-99-4859-8_6$2DOI
100 1 $aLACORTE, C. C.
245 $aSeed-based production of recombinant proteins.$h[electronic resource]
260 $c2023
300 $aCap. 6, p. 185-208.
520 $aThe use of plants to produce recombinant proteins has become a promising alternative to current expression systems based on microorganisms and cell cultures. Producing recombinant proteins in plants presents advantages such as reduced upstream costs, lower probability of infection by mammals’ pathogens, and easy scale-up production. Furthermore, plants can make posttranslational modifications and express large and complex proteins. Seeds stand out for their intrinsic characteristics among the plant tissues that can be used for recombinant protein production. In seeds, recombinant proteins are stored in protein storage vacuoles in the endosperm cells. They can be stably maintained for 4–6 years at room temperature without significant loss of protein and biological activity. Progress in methods for genome editing, alteration of the glycosylation pattern of the recombinant proteins produced, and use of protein fusions to aid downstream processes are some trends that shall contribute further to make molecular farming an attractive option for recombinant protein production. This chapter discusses the potential of seeds as a platform for producing recombinant proteins, across the scale-up of the production systems, basic approaches for the purification of recombinant proteins from plant cells, and biosafety issues
650 $aBiosafety
650 $aGlycosylation
653 $aForeign gene expression
653 $aFusion proteins
653 $aHeterologous protein production
653 $aPlant molecular farming
653 $aRecombinant protein purification
700 1 $aFERREIRA, A. L.
700 1 $aMURAD, A. M.
700 1 $aCUNHA, N. B. da
700 1 $aPINHEIRO, P. V.
773 $tIn: KOLE, C.; CHAURASIA, A.; HEFFERON, K. L.; PANIGRAHI, J. (ed.). Tools & techniques of plant molecular farming. [S.l.]: Springer Singapore, 2023. Cap. 6.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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| Acesso ao texto completo restrito à biblioteca da Embrapa Cerrados. Para informações adicionais entre em contato com cpac.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
09/03/1998 |
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Data da última atualização: |
09/03/1998 |
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Autoria: |
PIZARRO, E. A.; SILVA, G. P. da; SCHULTZE-KRAFT, R.; CORADIN, L. |
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Título: |
Areas de ocurrencia y recoleccion de germoplasma de Cratylia argentea en los Estados de Goias, Mato Grosso, Minas Gerais y Tocantins en Brasil. |
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Ano de publicação: |
1997 |
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Fonte/Imprenta: |
Pasturas Tropicales, Cali, v.19, n.1, p.10-15, 1997. |
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Idioma: |
Espanhol |
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Conteúdo: |
To identify new germplasm of the forage legume Cratylia argentea, a collection expedition was conducted in the states of Goias, Mato Grosso, and Tocantins in Brazil, where the center of diversity of the species is located. The populations of C. argentea varied in size, from isolated plants to populations of more than 500 plants. In dense populations, plant stems were thinner than those of isolated plans, which were sometimes more than 20 cm in diameter. The populations identified were located between 12 and 16 S, and between 46 and 58 W, 180 to 810 m above sea level, in soils that were mostly well-drained sandy loam, with variable chemical characteristics. Folowers were typically pink-violet (one population had white flowers) and fructification and seed production were abundant. Recovery of C. argentea plants after burning, cutting, or grazing was good, favored by this species's high leaf retention and tolerance of pests and diseases. |
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Palavras-Chave: |
Brasil; Goias; Mato Grosso; Minas Gerais; Tocantins. |
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Thesagro: |
Cerrado; Germoplasma. |
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Thesaurus NAL: |
Brazil; Cratylia argentea; germplasm. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01726naa a2200277 a 4500
001 1554081
005 1998-03-09
008 1997 bl --- 0-- u #d
100 1 $aPIZARRO, E. A.
245 $aAreas de ocurrencia y recoleccion de germoplasma de Cratylia argentea en los Estados de Goias, Mato Grosso, Minas Gerais y Tocantins en Brasil.
260 $c1997
520 $aTo identify new germplasm of the forage legume Cratylia argentea, a collection expedition was conducted in the states of Goias, Mato Grosso, and Tocantins in Brazil, where the center of diversity of the species is located. The populations of C. argentea varied in size, from isolated plants to populations of more than 500 plants. In dense populations, plant stems were thinner than those of isolated plans, which were sometimes more than 20 cm in diameter. The populations identified were located between 12 and 16 S, and between 46 and 58 W, 180 to 810 m above sea level, in soils that were mostly well-drained sandy loam, with variable chemical characteristics. Folowers were typically pink-violet (one population had white flowers) and fructification and seed production were abundant. Recovery of C. argentea plants after burning, cutting, or grazing was good, favored by this species's high leaf retention and tolerance of pests and diseases.
650 $aBrazil
650 $aCratylia argentea
650 $agermplasm
650 $aCerrado
650 $aGermoplasma
653 $aBrasil
653 $aGoias
653 $aMato Grosso
653 $aMinas Gerais
653 $aTocantins
700 1 $aSILVA, G. P. da
700 1 $aSCHULTZE-KRAFT, R.
700 1 $aCORADIN, L.
773 $tPasturas Tropicales, Cali$gv.19, n.1, p.10-15, 1997.
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